The Survival Rate, Fertilization and Developmental Capacity of Mature Mouse Oocyte Which Vitrified in Open Pulled Straw

Purpose: The aim of this study was to evaluate the effect of vitrification in open pulled straw on survival rate, fertilization and development capacity and comparison with conventional straw. Materials and Methods: NMRI mice were used for retrieval of oocytes. After administration of HMG and HCG, the mice scarified by cervical dislocation. Mice's abdomens were dissected and mature oocytes were delivered from fallopian tubes. Eighteen hundred & nintheen mature oocytes were allocated in three groups respectively as follow: I) 259 oocytes for vitrification in conventional straw, II) 283 oocytes for vitrification in open pulled straw and III) 277 oocytes without treatment (control group). All the oocytes were diluted in 0.5 mol sucrose solution. Results: The findings showed the, survival rate of oocytes in conventional straw was significantly lower than those using open pulled straw (73.8% Vs 84.8%) (P=0.001). Also fertilization rate of oocytes which vitrified thawed in conventional straw was significantly lower than those using open pulled straw (28.6% Vs 48.8%, P=0.000). Was significantly lower than those using open pulled straw. There was greater developmental capacity in oocytes were used open pulled straw than those treated in conventional straw (48.8% VS 24.3) (p<0.001). Conclusion: Open pulled straw technology has provided better capacity than conventional straw, but using this method in common remains elusive in IVF clinics.